ASCOT Library Zabali Campus

Master of Science in Agriculture (Crop Science)

A study on the response of mycelial growth and production efficiency of oyster mushroom (Pleurotus ostreatus) on varying levels of lime and different substrates was conducted at Aurora State College of Technology, Bazal Campus, Maria Aurora, Aurora from November 2020 to January 2021. The study aimed to determine the ideal lime percentage and substrate used for mycelial growth of oyster mushrooms.
The treatments were arranged in a factorial experiment in Complete Randomized Design (CRD). The experiment had twelve (12) treatments replicated three times. Analysis of Variance (ANOVA) was employed to test the overall significance of data while the least significant difference (LSD) test was sued to compare the differences among means. Factor A included the lime percentage such as Control (A1), 2% lime (A2), 4% lime (A3), and 6% lime (A4), while Factor B included the different substrate materials such as rice straw (B1), banana leaves (B2) and rice straw 70% + sawdust 30 % (B3). A total of 720 fruiting bags were used in the study.
The parameters used to evaluate the result were as follows: percentage of mycelial growth, days to complete mycelial growth, days to primordial initiation, days to harvest after primordial initiation, number of clusters/fruiting bags, total cap diameter, and total yield of harvest per treatment combination.
The analysis of variance exhibited significant to highly significant variation among all the parameters tested except for the percentage of mycelial growth on the first week and the fifth week. Based on the results of the study, on the third week of the percentage of mycelial growth, rice straw with 2% lime was the fastest to colonize, on the fourth week, the treatments that had reached 100% mycelial growth was obtained by control (RS 70% + S 30%), 2% (Banana leaves), 2% (RS 70% + S 30%), 4% lime, 4% (RS 70% + S 30%), 6% (Banana leaves) and 6 % (RS 70% + S 30%) while on the fifth week, all of the treatments obtained the 100% mycelial growth but showed no significant effects between the two factors. On the days to complete mycelial growth (21 days) and primordial initiation (2.17 days), rice straw with 2% lime obtained the shortest day.
Meanwhile, the days to harvest after primordial initiation (2 days) was obtained by 2% (RS 70% + S 30%) while the number of clusters/fruiting bags (9) was gained by the rice straw with 2% lime.
In terms of diameter of the cap, control (banana leaves) got the largest cap diameter (73.1mm) while the highest yield per treatment combination (61.40g) was recorded on the rice straw with a 2% lime percentage.